Home > Sandwich ELISA Kits > Mouse S100 Calcium Binding Protein A11 (S100A11) ELISA Kit

Product Name:

Mouse S100 Calcium Binding Protein A11 (S100A11) ELISA Kit

Cat.#:

JL24652

Regulatory Status:

RUO

Alternative:

S100-A11; S100C; MLN70; Protein S100-C; Calgizzarin; Metastatic lymph node gene 70 protein; Protein S100-A11, N-terminally processed

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.64ng/mL

Specificity:

This kit recognizes Mouse S100A11 in samples. No significant cross-reactivity or interference between Mouse S100A11 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| S100A11

S100 calcium-binding protein A11 (S100A11) is a protein that is encoded by the S100A11 gene.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100A11, also known as calgizzarin or 100C, is a small acidic protein. Along with all 13 members of the S100 family, are located as a cluster on chromosome 1q21.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	2.03	1.21	0.69	0.41	0.29	0.23	0.19	0.09
Corrected OD	1.94	1.12	0.6	0.32	0.2	0.14	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse S100A11 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	85-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	103

| Linearity

Samples were spiked with high concentrations of Mouse S100A11 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108